Composite
Part:BBa_K952007
Designed by: Saimon Sharif Group: iGEM12_Columbia-Cooper-NYC (2012-10-01)
IPTG induc. promoter-B0034-YF1-B0034-FixJ-PfixK2-Lysis Cassette
This part has the IPTG inducible promoter (BBa_J23102) upstream and BBa_K952002 downstream. BBa_K952002 originally did not have a promoter. This can be used immediately in a cell to cause lysis/cell death when a cell is exposed to blue-light and IPTG.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 790
Illegal NgoMIV site found at 862
Illegal NgoMIV site found at 952
Illegal NgoMIV site found at 970
Illegal NgoMIV site found at 1482
Illegal NgoMIV site found at 1775
Illegal NgoMIV site found at 1869
Illegal AgeI site found at 504
Illegal AgeI site found at 1650 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1539
Illegal BsaI.rc site found at 403
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Categories
Parameters
None |