Composite

Part:BBa_K952007

Designed by: Saimon Sharif   Group: iGEM12_Columbia-Cooper-NYC   (2012-10-01)


IPTG induc. promoter-B0034-YF1-B0034-FixJ-PfixK2-Lysis Cassette

This part has the IPTG inducible promoter (BBa_J23102) upstream and BBa_K952002 downstream. BBa_K952002 originally did not have a promoter. This can be used immediately in a cell to cause lysis/cell death when a cell is exposed to blue-light and IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 790
    Illegal NgoMIV site found at 862
    Illegal NgoMIV site found at 952
    Illegal NgoMIV site found at 970
    Illegal NgoMIV site found at 1482
    Illegal NgoMIV site found at 1775
    Illegal NgoMIV site found at 1869
    Illegal AgeI site found at 504
    Illegal AgeI site found at 1650
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1539
    Illegal BsaI.rc site found at 403


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Categories
Parameters
None